Premium
Sensitivity of Cultured Tissue of Arabidopsis thaliana Races to Sulfanilamide
Author(s) -
SCHOLL R. L.,
DENNISON K. M.
Publication year - 1978
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1978.tb01588.x
Subject(s) - sulfanilamide , callus , biology , dry weight , chemistry , biochemistry , botany
Callus of Arabidopsis thaliana (L.) Heynh. was examined for the kinetics of sulfanilamide inhibition of growth. Two geographic races of this species were compared for relative sulfanilamide sensitivity (as assessed by dry weight increase on drug‐containing medium) and rate of drug uptake. It was found that low concentrations of sulfanilamide in the culture medium (∼ 100 μ M ) inhibited callus growth and that low concentrations of p ‐aminobenzoate (20 μ M ) competitively antagonized the sulfanilamide effect. Racial variation for sulfanilamide sensitivity was demonstrated. A partially drug‐resistant race had greater sulfanilamide uptake (per gram fresh weight) than a sensitive race. suggesting that relative drug‐sensitivity was not determined by the capacity to exclude sulfanilamide.