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Changes in Malate Content and in Enzymes Involved in Dark CO 2 Fixation during Growth of Acer pseudoplatanus Cells in Suspension Culture
Author(s) -
VANDERHOVEN C.,
ZRŸUD J.P.
Publication year - 1978
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1978.tb01574.x
Subject(s) - phosphoenolpyruvate carboxylase , malic acid , enzyme , biochemistry , biology , logarithmic growth , pyruvate carboxylase , suspension culture , malic enzyme , botany , chemistry , cell culture , mathematical analysis , genetics , mathematics , citric acid , dehydrogenase
It has been reported in some cases that an increase in pCO 2 stimulates growth in diluted cell suspension cultures. Experiments have been designed to study the pattern of dark CO 2 fixation in sycamore cells grown in liquid suspension and to correlate this pattern with the culture growth phases. Comparisons were made between enzymatic activities, CO 2 incorporation, malic acid content during lag, logarithmic and stationary phases of growth. Malic enzyme (NADP‐dependent) was at its maximum activity during early logarithmic growth phase, when biosynthetic capacities were at the highest. Phosphoenolpyruvate‐carboxylase activity was strongly correlated with the ability of cells to fix CO 2 . Malic acid content decreased soon after transfer of the cells to a new medium and increased at the onset of stationary phase. Under optimal conditions, the CO 2 incorporation pattern did not change during growth, with an almost identical incorporation in the basic (amino acids) and acidic (organic acids) fractions. These observations have been discussed in relation to a possible effect of increased pCO 2 in the cell environment.