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Cytokinin Activity in Lupinus albus
Author(s) -
DAVEY J. E.,
STADEN J.
Publication year - 1978
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1978.tb01572.x
Subject(s) - lupinus , cytokinin , zeatin , anthesis , biology , point of delivery , botany , riboside , sephadex , biochemistry , auxin , cultivar , enzyme , gene
The cytokinins in the pod wall and seeds of the white lupin ( Lupinus albus L.) were investigated at 4, 6 and 8 weeks after anthesis. The level of cytokinin in the pod walls increased up to 8 weeks after anthesis, but then declined at 10 weeks. Fractionation on Sephadex LH‐20 shows that during the early stages of pod development cytokinin activity was associated mainly with substances co‐eluting with zeatin and zeatin riboside. However, in the later stages of pod wall growth an increase in activity was noted not only in these cytokinins but also in the cytokinins co‐eluting with the glucoside cytokinins. The level of cytokinin activity in the seeds decreased over the 10‐week experimental period. After fractionation on Sephadex LH‐20 four peaks of cytokinin activity were recorded in extracts of 6‐week‐old seeds. Two of these peaks co‐eluted with zeatin and zeatin riboside. The remaining peaks co‐eluted with the nucleotide and glucoside cytokinins. Only two peaks of activity co‐eluting with zeatin and zeatin riboside on Sephadex LH‐20 were recorded in 10‐week‐old seeds. Dihydrozeatin and its riboside also appear to be present in the seeds extracts. The significance of the quantitative and qualitative changes in the cytokinins of the seeds and pod walls is discussed.