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Response of Substituted Indoleacetic Acids in the Indolo‐α‐pyrone Fluorescence Determination
Author(s) -
BÖTTGER MICHAEL,
ENGVILD KJELD C.,
KAISER PETRA
Publication year - 1978
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1978.tb01567.x
Subject(s) - chemistry , fluorescence , pyrone , chromatography , organic chemistry , quantum mechanics , physics
The method of indolo‐α‐pyrone fluorescence‐determination of indoleacetic acid was investigated to study possible interference from 4‐chloro‐indoleacetic acid and 5‐hydroxyindoleacetic acid, which occur naturally. Both compounds show about 40% of the fluorescence of indoleacetic acid after conversion into their a ‐pyrones. Other halogenated indoleacetic acids show between zero and 60% of the fluorescence of indoleacetic acid. It is concluded that the concentration of indoleacetic acid cannot be determined in crude extracts in the presence of 4‐chloro‐ or 5‐hydroxy‐indoleacetic acid, because separate determinations of each of these compounds are not possible by changing the excitation or fluorescence wave‐lengths of the testing equipment.