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Connection between the Synthesis of Differentiation Specific Proteins and the Capacity of Cells to Respond to Cytokinin in the Moss Funaria
Author(s) -
BOPP MARTIN,
ERICHSEN UTA,
NESSEL MANFRED,
KNOOP BERND
Publication year - 1978
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1978.tb01542.x
Subject(s) - protonema , cytokinin , biology , microbiology and biotechnology , protein biosynthesis , moss , biochemistry , botany , auxin , gene
The differentiation stage of the caulonema in Funaria hygrometrica protonema is distinguished from the chloronema stage by three additional protein bands (CSP) in the soluble protein fraction. During the change of caulonema to chloronema, which is induced by isolation of single filaments (regeneration), the CSP disappear. This is not the consequence of an accelerated degradation or turnover but of a gradual termination in the de novo synthesis of CSP during regeneration as demonstrated by pulse‐chase experiments with l ‐[4,5– 3 H] leucine. Cytokinin inhibits the termination of the synthesis of CSP. The decrease in synthesis parallels the decrease in ability of the isolated caulonema cells to respond to cytokinin via bud formation. It is therefore concluded that the CSP are involved in the competence of caulonema cells to respond to cytokinins.