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Influence of Phytohormones on the Response of Cultured Cotton Ovules to (2‐chloroethyl)phosphonic Acid
Author(s) -
STEWART JAMES McD.,
HSU CECILIA LEE
Publication year - 1977
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1977.tb09288.x
Subject(s) - callus , kinetin , ovule , gibberellic acid , abscisic acid , biology , botany , integument , tissue culture , horticulture , in vitro , biochemistry , germination , pollen , gene
Callus was induced from the micropylar end of cultured cotton ( Gossypium hirsutum L.) ovules by (2‐chloroethyl)phosphonic acid (CEPA). The influence of other phytohormones on the growth of this callus was examined with in vitro culture techniques. Abscisic acid inhibited the callus growth induced by 10 −4 M CEPA, but at low concentrations of abscisic acid and CEPA, callus growth was stimulated slightly. Indoleacetic acid inhibited the callus growth at optimum CEPA concentration, but not when the CEPA level was supraoptimum. Kinetin stimulated micropylar callus growth when ovules were obtained from parent plants grown under long, warm days but not when ovules were from plants grown under short days and cooler temperatures. Gibberellic acid (GA) caused the greatest callus response. Increasing concentrations of GA up to 5 × 10 −7 M markedly stimulated callus growth while increasing the sensitivity of the tissue to CEPA. In addition to induction and growth of the micropylar callus, combinations of CEPA and GA caused a complete release of normal tissue differentiation in the cotton ovule with rapid proliferation of the entire ovule integument. This is a promising system with which to study hormonal control of tissue differentiation.