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Partial Synchronization of Carrot Cell Culture by Auxin Deprivation
Author(s) -
NISHI ARASUKE,
KATO KENJI,
TAKAHASHI MASAO,
YOSHIDA RYUJI
Publication year - 1977
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1977.tb09277.x
Subject(s) - auxin , daucus carota , cell division , mitosis , biology , doubling time , dna synthesis , botany , cell culture , contact inhibition , cell , microbiology and biotechnology , biochemistry , dna , genetics , gene
A strain of carrot cells ( Daucus carota cv. Kintoki) grew exponentially in the presence of 2,4‐dichlorophenoxyacetic acid (2,4‐D, 1 mg/1) with a doubling time of about 2 days. When those cells were transferred to a medium lacking 2,4‐D, they continued to grow at almost the same rate for about a week. When the cells were again transferred to the auxin‐free medium, the rate of cell division gradually decreased. After the cell division had ceased, cells were returned to the ordinary 2,4‐D medium. A burst of cell divisions occurred after about 2 days. Timing of DNA synthesis and of mitosis suggested that the cells had been arrested at G 1 phase. In a medium containing indoleacetic acid instead of 2,4‐D, the auxin was rapidly degraded and the culture was similarly synchronized as in the auxin‐omitted medium.