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Tissue Culture of Maize
Author(s) -
SHERIDAN WILLIAM F.
Publication year - 1977
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1977.tb05553.x
Subject(s) - callus , strain (injury) , zea mays , amino acid , seedling , basal (medicine) , biology , poaceae , tissue culture , horticulture , botany , food science , biochemistry , in vitro , agronomy , microbiology and biotechnology , insulin , anatomy
Callus of 12 strains of maize ( Zea mays L.) was grown on a basal medium and on media supplemented singly with 0.3, 1.0 or 3.0 m M of either the glutamate, aspartate, or aromatic families of amino acids. Groth rate on basal medium difrered significantly among strains with Black Mexican Sweet corn growing most rapidly. Although there were three cases of simulation of callus growth at the 0.3 m M level and one case at the 1.0 m M level, generally the addition of amino acid families had either no effect or were inhibitory to growth rate. The sensitivity of inhibition varied among the strains tested at the 3.0 m M level except for the strain Ibadan B. Strains of the sweet, flint, pop and dent types of maize were all represented among the more fapidly growing strains and among those significantly inhibited by inclusion of amino acids in the cultural media. It remains to be determined as to whether the differences in growth response of the callus from the different strains is strain specific since, in all cases but one, callus was obtained from a single seedling of each strain.