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Pretreatment of Cell Suspensions as a Method to Increase the Protoplast Yield of Haplopappus gracilis
Author(s) -
WALLIN ANITA,
GLIMELIUS KRISTINA,
ERIKSSON TAGE
Publication year - 1977
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1977.tb04078.x
Subject(s) - protoplast , cellulase , pectinase , yield (engineering) , suspension culture , sugar , auxin , chemistry , botany , cell wall , chromatography , biology , horticulture , biochemistry , cell culture , enzyme , materials science , gene , metallurgy , genetics
A low yield of free protoplasts was obtained when fast‐growing cell suspensions of Haplopappus gracilis were treated with Driselase, an active mixture of cellulase and pectinase. If the cells in the suspension culture were pretreated by increased levels of auxin, reduced sugar concentration, addition of sulphur‐containing amino acids, or by the reducing agent mercaptoethanol, protoplasts were released from a higher proportion of the cells. These pretreatments did not adversely effect division of the protoplasts.