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Cellulolytic Enzyme System in Culture Filtrates of Aspergillus sydowi
Author(s) -
OLUTIOLA P. O.,
COLE O. O.
Publication year - 1977
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1977.tb04045.x
Subject(s) - cellulase , size exclusion chromatography , enzyme , hydrolysis , chromatography , cellulose , chemistry , cellulosic ethanol , enzymatic hydrolysis , aspergillus , biochemistry , fraction (chemistry) , biology , microbiology and biotechnology
During growth in liquid culture medium, that contained single soluble or insoluble cellulosic carbon source, Aspergillus sydowi (Bain. & Start.) Thom & Church released cellulolytic enzymes into the medium. The enzymes were separated by gel filtration followed by ion exchange chromatography into three components, all of high molecular weight. One of the components (Ac) has the character of a C 1 cellulase enzyme. In the assay for hydrolysis of insoluble cellulose, the combined fractions, especially whenever the fraction under test contained the component Ac, released more glucose than when each component was employed alone.