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Improved Growth of Tissue Cultures of the Onion, Allium cepa
Author(s) -
DUNSTAN D. I.,
SHORT K. C.
Publication year - 1977
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1977.tb01525.x
Subject(s) - allium , callus , suspension culture , tissue culture , ammonium , phosphate , horticulture , botany , dry weight , biology , food science , chemistry , cell culture , biochemistry , in vitro , organic chemistry , genetics
A forty‐fold increase in tissue fresh weight has been achieved for Allium cepa stem tissue callus grown on a modified B5 medium with increased ammonium, phosphate and nitrogen levels; and decreased level of 2,4‐dichlorophenoxyacetic acid. Growth was examined on several standard media, using fresh weight, dry weight and cell number as parameters over an 8‐week growth period. Further, tissue grown upon this medium is more friable than on other media. The value of such friable callus for suspension culture initiation and for single cell cloning experiments is discussed.