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Dipeptides and Amino Acids as Nitrogen Sources for the Callus of Atropa belladonna
Author(s) -
SALONEN MAIJALIISA,
SIMOLA LIISA KAARINA
Publication year - 1977
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1977.tb01522.x
Subject(s) - proline , glycine , callus , amino acid , valine , methionine , alanine , hydroxyproline , chemistry , nitrogen , atropa belladonna , tyrosine , biochemistry , arginine , leucine , stereochemistry , botany , biology , organic chemistry
Stem callus of Atropa belladonna was grown on media containing seven dipeptides and the corresponding amino acids, given as the only nitrogen sources and in combination with inorganic nitrogen. Of the eight amino acids studied, only alanine and proline were used effectively by the callus. Three dipeptides (glycyl‐alanine, glycyl‐glycine and prolyl‐proline) promoted growth slightly, but none of them was better than inorganic nitrogen (KNO 3 , 2.5 m M ) as the sole nitrogen source. In combination with KNO 3 (2.5 m M ), these three peptides stimulated growth more than, or at least as much as, KNO 3 (5 m M ). Growth was better with glycyl‐alanine and glycyl‐glycine (1.25 m M ) than with glycine (2.5 m M ), which inhibited growth. Arginine, a usually favourable compound, did not give any growth when used as the sole nitrogen source. Leucyl‐tyrosine, prolyl‐hydroxyproline and prolyl‐methionine inhibited growth when given alone or with inorganic nitrogen. In the case of the prolyl peptides the inhibition seemed to be due to the unfavourable effects of methionine and hydroxy‐proline, which were not counteracted by an equimolar concentration of proline. Both leucine and tyrosine were also strongly growth‐retarding.