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Changes in Metabolite Levels during Growth of Acer pseudoplatanus (Sycamore) Cells in Batch Suspension Culture
Author(s) -
SHIMIZU TOMIKO,
CLIFTON ANNE,
KOMAMINE ATSUSHI,
FOWLER MICHAEL W.
Publication year - 1977
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1977.tb01508.x
Subject(s) - acer pseudoplatanus , phosphofructokinase , pyruvate kinase , glycolysis , metabolite , energy charge , biochemistry , chemistry , suspension culture , suspension (topology) , enzyme , botany , chromatography , biology , cell culture , mathematics , homotopy , pure mathematics , adenylate kinase , genetics
Measurements were made of the levels of key glycolytic intermediates and co‐factors during the growth of Acer pseudoplatanus L. cells in batch culture. Mass action ratios were calculated for the enzymes phosphoglucoseisomerase, phosphofructokinase and pyruvate kinase. The ratio for phosphofructokinase was greatly displaced from equilibrium. Major increases in the level of NADPH and ATP and energy charge were observed in the initial or lag period of culture. The data indicate that the energy generating system of the cells is most active in the lag phase.