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Rise in the Ratio Heavy to Light Extractable Ribosomal RNA during Sprouting of Birdcherry Buds
Author(s) -
EKELUND R.
Publication year - 1977
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1977.tb01506.x
Subject(s) - ribosomal rna , sprouting , nucleic acid , rna , biology , sucrose , centrifugation , prunus , botany , biochemistry , gene
Using the diethyl pyrocarbonate method, nucleic acids have been extracted from buds of European bird‐cherry ( Prunus padus L.) in different developmental stages and analysed by sucrose gradient centrifugation. The ratio heavy to light ribosomal RNA was about I in extracts from dormant and non‐dormant but slowly growing buds. In extracts from sprouting buds the ratio was about 1.7. This increase probably reflects an increased extractability of heavy ribosomal RNA and may depend on a change of ribosomal structure during sprouting.