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Photoautotrophic Growth and Photosynthesis in Cell Suspension Cultures of Chenopodium rubrum
Author(s) -
HÜSEMANN WOLFGANG,
BARZ WOLFGANG
Publication year - 1977
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1977.tb01498.x
Subject(s) - photosynthesis , chlorophyll , suspension culture , chlorophyll a , botany , biology , horticulture , chemistry , food science , cell culture , genetics
A method is described for growing cell suspension cultures of Chenopodium rubrum photoautotrophically for prolonged periods of time. By using a two‐tier culture vessel the growth medium with the cells was separated from the CO 2 reservoir. Definite CO 2 concentrations were established by a K 2 CO 3 /KHCO 3 buffer. Photoautotrophic growth in C. rubrum cell suspension cultures was correlated with the CO 2 level. At 0.5% CO 2 the cell cultures contained 68 μg chlorophyll/g fresh weight and showed an increase in fresh weight of about 80% in 18 days. At 1% CO 2 an increase in fresh weight of 165% in 18 days was observed. The chlorophyll content rose up to 84 μg/g fresh weight. The photoautotrophic growth was also greatly influenced by the 2,4‐D content of the medium. Cell growth was enhanced by lowering the auxin concentration. Best growth was attained (210% increase in fresh weight) at 10 −8 M 2,4‐D. The photosynthetic activity of the cells was measured by the light dependent 14 CO 2 incorporation. At 0.5% CO 2 the cell suspensions assimilated about 100 μmol CO 2 /mg chlorophyll × h. In the presence of 1% CO 2 the light driven assimilation was raised up to 185 μmol CO 2 /mg chlorophyll × h. In both cases, the dark incorporation of CO 2 was merely 1.8% of the values obtained in light.