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The Intracellular Location of Abscisic Acid in Stressed and Non‐Stressed Leaf Tissue
Author(s) -
LOVEYS B. R.
Publication year - 1977
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1977.tb01483.x
Subject(s) - abscisic acid , chloroplast , lysis , spinach , chemistry , intracellular , plastid , in vitro , biochemistry , botany , biology , gene
The intra‐cellular location of ABA was investigated in relation to its sites of synthesis. Chloroplasts were isolated from stressed and non‐stressed spinach leaves and their ABA content determined. Virtually all of the ABA from non‐stressed leaves was contained in the chloroplasts compared with only a small fraction of ABA isolated from stressed leaves. Chloroplasts prepared from turgid leaves and subsequently lysed in vitro retained most of their ABA and phaseic acid (PA) complement but this was removed with organic solvents. While the possibility of extra‐chloroplastic synthesis cannot be discounted the data indicate that stress‐induced ABA synthesis occurs in the chloroplast and that the ABA readily migrates from there to other parts of the plant.