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Uptake of 14 C‐L‐Glutamic Acid by Daucus carota Cell Suspension in Different Shock Situations
Author(s) -
WAHLSTRÖM DAN,
ERIKSSON TAGE
Publication year - 1976
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1976.tb04873.x
Subject(s) - daucus carota , sucrose , inoculation , polyethylene glycol , glutamic acid , chemistry , suspension (topology) , calcium , suspension culture , chromatography , nuclear chemistry , botany , horticulture , biochemistry , cell culture , biology , amino acid , organic chemistry , genetics , mathematics , homotopy , pure mathematics
Cell suspension cultures of Daucus carota were shocked by inoculation or by temperature, salts or osmotica in combination with inoculation. Changes in cell vitality were measured by uptake studies with labelled L‐glutamic acid performed during the first 30 min after shock treatment. The cell sample was then collected on filter and counted in a liquid scintillation counter. Inoculation alone caused a marked decrease in uptake. Normal uptake capacity was re‐established within 2 h. Temperatures of 0−20°C as against 27°C gave an additional decrease in uptake. Of the tested osmotica, polyethylene glycol gave the greatest stabilizing effect on the glutamic acid uptake and sucrose the least. The highest uptake rates were obtained with a calcium concentration of 0.10–0.15 m M .