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Embryogenesis and Differentiation in Nigella sativa Leaf Callus in vitro
Author(s) -
BANERJEE SABITA,
GUPTA SUKUMAR
Publication year - 1976
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1976.tb04869.x
Subject(s) - callus , kinetin , somatic embryogenesis , botany , hydrolysate , nigella sativa , biology , meristem , subculture (biology) , murashige and skoog medium , tissue culture , shoot , casein , in vitro , traditional medicine , biochemistry , medicine , hydrolysis
Embryogenesis occurred in Nigella sativa L. (Fam. Ranunculaceae) leaf callus tissue when coconut milk was replaced from the Murashige and Skoog's (MS) medium by casein hydrolysate. On MS + IAA (0.5 mg/l) + casein hydrolysate (100 and 500 mg/l) medium, tissue gained a capacity of growing embryoids for a pro‐longed culture period. At a concentration of 1000 mg/l casein hydrolysate suppressed the differentiating capacity after the fifth subculture. 2.4‐D and kinetin had inhibitory effects on morphogenesis. Histology of the differentiated tissue revealed that the origin of roots, shoot buds and leaves were from groups of meristematic cells whereas embryoids were initiated by the repeated division of a single cell.