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Characterization of the Aminoacylation of Cytoplasmic Transfer Ribonucleic Acids from Germinating Soybeans
Author(s) -
TESTER C. F.
Publication year - 1976
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1976.tb04421.x
Subject(s) - aminoacylation , spermidine , glycine , pyrophosphate , chemistry , germination , magnesium , transfer rna , biochemistry , in vitro , spermine , rna , amino acid , biology , botany , enzyme , organic chemistry , gene
Extracts from germinating soybean [ Glycine max (L.) Merr.] cotyledons were assayed for aminoacyl‐tRNA formation. This esterification reaction depends on several factors, which must be determined and controlled for each system investigated. The reaction was maximum when the Mg: ATP ratio was 12.5 and concentrations were 9 and 0.72 m M , respectively. The optimum temperature for the in vitro reaction was 25°C. In addition, the reaction was inhibited by inorganic pyrophosphate and magnesium could be replaced by spermidine.