Premium
Uptake of Gibberellic Acid in Intact and Scarified Seeds of Barbarea vulgaris
Author(s) -
TAYLORSON R. B.
Publication year - 1976
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1976.tb03991.x
Subject(s) - gibberellic acid , germination , hypocotyl , gibberellin , biology , horticulture , botany , chemistry , agronomy
Uptake of gibberellic acid as a function of duration of exposure, external concentration, and seed lot was measured in seeds of yellow rocket ( Barbarea vulgaris R. Br.) by means of lettuce hypocotyl bioassays of the acidic, basic, or neutral fractions of seed extracts and by uptake of 14 C‐GA 3 . In intact seeds, where m M levels of GA 3 promoted only 25% germination, uptake was completed within 24 h of exposure. The maximum uptake was about 0.2% of external amount. Although germination promotion by GA 3 differed among seedlots of yellow rocket, relative uptake (percentage of the external GA 3 ) was nearly the same. The relative rate of uptake of GA 3 was similar for scarified and intact seeds, but germination was promoted in scarified seeds by much lower levels of GA 3 than in intact seeds. Total uptake in scarified seeds was much higher, however (about 10% of the external amount). In seeds imbibed in H 2 O, practically no endogenous GA‐like activity was detected in either the acidic, basic, or neutral fractions. It was also apparent that intact seeds could take up quantities of GA 3 that failed to promote germination, but were comparable to quantities that promoted germination in scarified seeds.