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Regeneration of Plants from Cell Suspensions Frozen at −20, −70 and −196°C
Author(s) -
BAJAJ Y. P. S.
Publication year - 1976
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1976.tb03968.x
Subject(s) - datura , callus , liquid nitrogen , plant cell , cell wall , cell , botany , protoplast , daucus carota , regeneration (biology) , biology , horticulture , chemistry , microbiology and biotechnology , biochemistry , gene , organic chemistry
Cell suspensions of carrot, Datura , tobacco and soybean subjected to −20°C, −70°C and −196°C in the presence of a suitable cryoprotective agent, and stored for various lengths of time have been revived. After revival these cells divided to form callus masses. Direct immersion in liquid nitrogen invariably killed the cells, whereas cooling at the rate of 1 or 2°C/min, or pre‐freezing briefly at −20 and −70°C, followed by freezing at −196°C retained the viability. Depending on the plant species up to 70% of the cell clumps could withstand ultra‐cooling. Tobacco and Datura cell suspensions were more sensitive to cold treatment than were those of carrot. Actively growing cell suspensions containing small cell‐clumps revived rapidly, while filtered cell‐suspensions of free cells only occasionally survived. Calli of tobacco and carrot obtained from frozen suspensions have been regenerated into plants.