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Environmentally Induced Changes in the Cell Walls of Tomato Leaves in Relation to Cell and Protoplast Release
Author(s) -
CASSELLS ALAN C.,
BARLASS MARTIN
Publication year - 1976
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1976.tb03964.x
Subject(s) - protoplast , pectinase , cellulase , cell wall , biology , pectin , botany , sodium citrate , pectate lyase , enzyme , biochemistry , medicine , pathology
Factors involved in the isolation of protoplasts from the leaves of tomato plants grown over a wide range of environmental conditions have been studied. Increases in calcium pectate in summer grown (“hard”) plants are suggested as a barrier to cell wall degradation. A one‐step method involving the addition of sodium citrate to pectinase plus cellulase gives high yield of protoplasts from hard plants. Attempts to convert isolated palisade cells to protoplasts have failed. The plant culture conditions are described such that protoplasts may be isolated throughout the year using low enzyme concentrations.