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Carbon Dioxide as an Essential Requirement for Cultured Sycamore Cells
Author(s) -
GATHERCOLE R. W. E.,
MANSFIELD K. J.,
STREET H. E.
Publication year - 1976
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1976.tb03960.x
Subject(s) - carbon dioxide , amino acid , suspension culture , agar , suspension (topology) , chemistry , carbon fibers , botany , biochemistry , cell culture , biology , organic chemistry , materials science , bacteria , mathematics , homotopy , composite number , pure mathematics , composite material , genetics
Carbon dioxide (optimum concentration c. 1.0%) is essential to the initiation of the growth in suspension culture or on agar plates of cultured sycamore cells. By effective flushing of the cultures with CO 2 ‐free air it is possible to demonstrate this requirement with initial cell densities up to 50 × 10 3 cells ml −1 . This growth‐promoting activity of carbon dioxide is not related to any effect it may have on the pH of the culture medium. The cells fix applied carbon dioxide into organic and amino acids but attempts to replace the carbon dioxide requirement by non‐toxic levels of organic or amino acids have not been successful.