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Properties of Phenylalanyl‐tRNA Synthetase and Phenylalanine Ammonia‐lyase of Pine Suspension Cultures
Author(s) -
COWLES JOE R.,
FOWLER JO ANN
Publication year - 1976
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1976.tb03946.x
Subject(s) - phenylalanine , phenylalanine ammonia lyase , enzyme , ammonia , biochemistry , transfer rna , yeast , chemistry , suspension culture , biology , amino acid , rna , cell culture , genetics , gene
Phenylalanyl‐tRNA synthetase and phenylalanine ammonia‐lyase activities were demonstrated in partially purified extracts of pine ( Pinus elliottii ) suspension cultures. The optimum pH for the phenylalanyl‐tRNA synthetase reaction was 7.5 and the optimum ATP and Mg 2+ concentrations were 1.0 and 15 m M respectively. Pine, calf liver and yeast tRNA were inadequate substitutes for pea tRNA in the synthetase reaction mixtures. The optimum pH for the phenylalanine ammonia‐lyase reaction was 9.0. The K m for phenylalanine was approximately 6.6 × 10 −5 M . The activity of both enzymes in the partially purified extracts was unstable on storage.