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Respiration and NADH‐Oxidation of the Roots of Flood‐Tolerant and Flood‐Intolerant Senecio Species as Affected by Anaerobiosis
Author(s) -
LAMBERS HANS
Publication year - 1976
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1976.tb03943.x
Subject(s) - nitrate reductase , senecio , anaerobic exercise , respiration , dehydrogenase , glutamate dehydrogenase , biology , lactate dehydrogenase , nadh dehydrogenase , cellular respiration , glutamate synthase , reductase , anaerobic respiration , enzyme , biochemistry , botany , glutamate receptor , physiology , receptor , protein subunit , gene
Respiration was measured under anaerobiosis in the roots of two Senecio species: S. aquaticus Hill, which is flood‐tolerant, and S. jacobaea L., which is flood‐intolerant. NADH‐oxidation under anaerobiosis was measured in roots of S. aquaticus, S. jacobaea and S. vulgaris L., which is also flood‐intolerant. Protein content of S. aquaticus was about 15% higher under anaerobiosis. At 20°C respiration of the roots of S. aquaticus was 50% inhibited under anaerobiosis, while an almost complete inhibition occurred in the roots of S. jacobaea . The activities of nitrate reductase, glutamate dehydrogenase and lactate dehydrogenase were considerably higher in the roots of S. aquaticus grown under anaerobic conditions than in roots grown under aerobic conditions. In S. jacobaea glutamate dehydrogenase activity was lower and in S. vulgaris nitrate reductase was lower and glutamate dehydrogenase activity was higher in roots grown under anaerobic conditions. The possible role of these enzymes for metabolism under anaerobic conditions by oxidizing a surplus of NADH is discussed. Since oxidative phosphorylation is 50% inhibited under anaerobiosis, ATP has to be generated in a different way. It is argued that maintenance of the ATP‐level may be compensated by way of the enzymes mentioned above, in combination with a modified glucose utilization.