Premium
The Influence of 8‐Hydroxyquinoline on the Accumulation of Porphyrins in Dark‐Grown Wheat Leaves Treated with δ‐Aminolevulinic Acid
Author(s) -
RYBERG MARGARETA,
SUNDQVIST CHRISTER
Publication year - 1976
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1976.tb02256.x
Subject(s) - protochlorophyllide , 8 hydroxyquinoline , chemistry , chlorophyll , metabolism , phosphate buffered saline , biochemistry , nuclear chemistry , biosynthesis , inorganic chemistry , chromatography , enzyme , organic chemistry
Dark‐grown wheat leaves treated with δ‐aminolevulinic acid and 8‐hydroxyquinoline accumulated porphyrins, most of which were protochlorophyllide and magnesiumprotoporphyrin monomethylester. The ratio between these two components was dependent on the concentration of 8‐hydroxyquinoline. Small amounts of other porphyrins could also be detected. The treatment with 8‐hydroxyquinoline and the presence of large amounts of porphyrins other than protochlorophyllide did not influence the photoreduction of protochlorophyllide or the Shibata shift. 8‐Hydroxyquinoline caused an inhibition of protochlorophyllide biosynthesis, which could be reversed by rinsing the leaves several times with phosphate buffer. Magnesiumprotoporphyrin monomethylester was then converted to protochlorophyllide. The reversal induced by washing was increased if the buffer contained iron. The possible function of iron in the chlorophyll metabolism and its role in the inhibition reactions with 8‐hydroxyquinoline are discussed.