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Light‐Induced Absorption Changes in Etiolated Coleoptiles
Author(s) -
WIDELL S.,
BJÖRN L. O.
Publication year - 1976
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1976.tb02247.x
Subject(s) - etiolation , coleoptile , absorbance , absorption (acoustics) , iodide , chemistry , photochemistry , pulse (music) , blue light , analytical chemistry (journal) , materials science , biophysics , optics , biology , chromatography , inorganic chemistry , biochemistry , physics , optoelectronics , detector , composite material , enzyme
Blue (or green) light induced reversible and irreversible absorption changes in etiolated wheat ( Triticum aestivum ) coleoptiles. Immediately after a 10 s pulse of blue light there was an absorbance increase at 440 nm and a decrease at 420 nm. The absorbance at 440 nm returned to the original level in a biphasic way, with first‐order rate constants of 0.05 s −1 and 0.006 s −1 at 25°C. The change at 440 nm was partly, but not completely, inhibited by iodide. In the 500–600 nm region complex changes occurred, indicating the participation of at least two different cytochromes, one of which was oxidized during a 10 s light pulse and the other oxidized more slowly during the following dark period.