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The Inhibitory Action of Cycloheximide on Respiration of Coleoptiles is Relieved by Light
Author(s) -
DHEIDAH M.,
BLACK M.
Publication year - 1976
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1976.tb01877.x
Subject(s) - coleoptile , cycloheximide , darkness , avena , respiration , botany , oxygen , biology , biochemistry , biophysics , chemistry , protein biosynthesis , organic chemistry
Concentrations of cycloheximide which are frequently used for the specific purpose of inhibiting protein synthesis also reduce oxygen uptake in Triticum and Avena coleoptiles in darkness by up to 50%. Except at high concentrations of the chemical diffuse daylight almost completely relieves this inhibitory effect provided the coleoptiles are not submerged. In darkness oxygen consumption is rapidly affected when cycloheximide is supplied to coleoptiles; the relief by light is also fast. These observations raise questions concerning the use of cycloheximide in studies with coleoptiles.