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The Potassium Requirement for Growth and Embryogenesis in Wild Carrot Suspension Cultures
Author(s) -
BROWN SUSAN,
WETHERELL DONALD F.,
DOUGALL DONALD K.
Publication year - 1976
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1976.tb01875.x
Subject(s) - daucus carota , embryogenesis , embryo , potassium , ammonium , biology , nitrogen , suspension culture , chemistry , botany , cell culture , microbiology and biotechnology , genetics , organic chemistry
A requirement for potassium for growth and forembryogenesis in suspension cultures of wild carrot ( Daucus carota L.) was demonstrated. The concentration of K + required for maximal growth (1 m M ) was less than that required for maximal embryogenesis (20 m M ). Neither Na + nor NH 4 + could replace K + . Ammonium ion enhanced embryogenesis when K + was present at suboptimal levels greater than 1 m M . Nitrogen sources strongly influenced growth and embryogenesis, but the effects of nitrogen were separable from those of K + . Subline differences were noted. Subline CSC‐29 produced nearly half the maximum embryo number in 1 m M K + while CSC‐31 produced no embryos at that K + concentration. Growth of CSC‐29 was slightly repressed by Na + , but no more than by similarconcentrations of K + . Growth of CSC‐31 in 1 mM K + was strongly repressed by Na + . Embryogenesis in CSC‐29 was unaffected by Na + . In CSC‐31, Na + repressed embryogenesis at lower concentrations of K + .