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Comparative Study of β‐Glucosidase from Cotyledons and Fruits of Cucumber, Cucumis sativus
Author(s) -
McCREIGHT J. D.,
PHARR D. M.,
LOWER R. L.,
SOX H. N.
Publication year - 1976
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1976.tb01866.x
Subject(s) - cucumis , cotyledon , enzyme , glucosidases , enzyme assay , michaelis–menten kinetics , biology , botany , chemistry , horticulture , biochemistry
This study was conducted to compare the β‐glucosidase of cotyledons and fruits of Cucumis sativus L. cv. Chipper. The concentration of the enzyme was followed throughout the growth period of each organ. The greatest concentration of the enzyme did not correspond with the most rapid period of growth. Each enzyme was characterized kinetically. The Michaelis constant of the cotyledon β‐glucosidase for p ‐NO 2 ‐phenyl‐β‐D‐gluco‐pyranoside was 1.57 mM, and was 0.35 mM for the fruit enzyme. The enzymes from the two sources also differed in affinity for glucono‐1,5‐lactone, a competitive inhibitor of β‐glucosidases, susceptibility to inhibition by saccharides, and heat stability. The two organs apparently contain different forms of β‐glucosidase.