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Recovery of Cytokinins from Cation Exchange Resins
Author(s) -
VREMAN HENDRIK J.,
CORSE JOSEPH
Publication year - 1975
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1975.tb03916.x
Subject(s) - elution , chemistry , zeatin , chromatography , ethanol , polystyrene , ion exchange resin , matrix (chemical analysis) , ion exchange , nuclear chemistry , cytokinin , organic chemistry , biochemistry , ion , auxin , gene , polymer
Cytokinins exhibit great affinity for the polystyrene matrix of Dowex‐50 cation exchange resins. Model experiments showed that only c . 20% of the applied 2‐methylthio‐ trans ‐zeatin and c . 70% of the trans ‐zeatin are eluted from AG 50W columns with six bed volumes of 1 N NH 4 OH. Inclusion of 70% (v/v) ethanol in the eluant increases the recovery to c . 55 and 85% respectively. No additional recovery was obtained upon continued elution to 14 bed volumes. Similar experiments with Duolite CS‐101, a weak acid cation exchange resin with acrylic matrix, showed that c . 95% of the applied 2‐methylthio derivatives of isopentenyladenine and zeatin are recovered from the resin with six bed volumes of 70% (v/v) ethanol (pH 10) or 1 N NH 4 OH in 70% (v/v) ethanol. The less modified cytokinins, isopentenyladenosine and ribosylzeatin, are recovered completely under these conditions. Recovery of cytokinins was determined by means of high‐pressure liquid chromatography of the column eluates.