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Influence of S‐ethyl dipropylthiocarbamate (EPTC) on Wheat Root Phospholipid Fatty Acid Composition
Author(s) -
KARUNEN P.,
WILKINSON R. E.
Publication year - 1975
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1975.tb03898.x
Subject(s) - chemistry , fatty acid , linolenic acid , phospholipid , palmitic acid , linoleic acid , composition (language) , fraction (chemistry) , food science , gas chromatography , dry weight , chromatography , botany , biochemistry , biology , linguistics , philosophy , membrane
Winter wheat ( Triticum sativum L. ev. Nisu) was grown in sand which contained 0, 0.25, 0.5 and 1.0 mg S‐ethyl dipropylthicarbamate (EPTC) per kg air dry sand. In 21 day old roots, the phospholipids (PL) were extracted in ice‐cold chloroform/methanol (2:1, v/v) and isolated by thin layer chromatography (TLC). The PL fatty acids were analysed by gas liquid chromatography (GLC). The major fatty acids of the root PL fraction were palmitic, oletic, linoleic, and linolenic (22.4, 6.8, 39.2, and 23.1 μ/g root fresh weight, respectively). Total fatty acid content of the PL fraction was decreased to 39% by 1 mg EPTC/kg sand in which linolenic acid was decreased to 28%. The remainder of the major fatty acid constituents were decreased to 12–47%. The general quality of fatty acids in the PL fraction was slightly altered, while a 60% inhibition of total PL production resulted.