Premium
Tracheary Element Formation from Single Isolated Cells in Culture
Author(s) -
TORREY JOHN G.
Publication year - 1975
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1975.tb03886.x
Subject(s) - petri dish , biology , callus , tissue culture , cell division , suspension (topology) , suspension culture , botany , cell culture , cellular differentiation , parenchyma , microbiology and biotechnology , cell , biochemistry , in vitro , genetics , mathematics , homotopy , gene , pure mathematics
Friable callus tissue of Centaurea cyanus L. was grown on a solidified synthetic nutrient medium (EBM‐1) to produce a tissue with a low frequency of differentiated tracheary elements. Tissues were then suspended in liquid nutrient medium with agitation to produce a suspension which was filtered and the single‐cell suspension resulting was used as inoculum for either cell suspension cultures or for plating of cells into solidified medium in Petri plates. Media for the suspension cultures were selected to favor cytodifferentiation of tracheary elements. Differentiated tracheary elements formed as early as 10 days and numbers of tracheary elements increased with time roughly in relation to the increase in total cell number. From plating experiments it was shown conclusively that single isolated parenchyma cells differentiated directly into single isolated tracheary elements, although this event was rare. More usual was the division of isolated cells to form small colonies and then the differentiation of one, several or all of the cells into tracheary elements. Comparisons are made between results with cell plating experiments and cell suspension cultures. Optimism is expressed for finding a cell suspension culture system for studying cytodifferentiation.