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Effect of Na + , K + , Mg 2+ and ATP on the Relative Electrophoretic Mobility of Sugar Beet Membrane Particles with NaK ATPase Activity
Author(s) -
KARLSSON JAN
Publication year - 1975
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1975.tb03871.x
Subject(s) - membrane , chemistry , tris , atpase , pi , sugar beet , electrophoresis , membrane transport , chromatography , biochemistry , nuclear chemistry , enzyme , biology , horticulture
Electrophoretic measurements on membrane coated particles were performed with a Zytopherometer. Tris‐HCl buffer 0.2 M pH 7.0 at 37°C with addition of different combinations of Na + , K + , Mg 2+ and ATP was used as test medium. The membranes were of two types, an untreated preparation with low NaK ATPase activity and a deoxycholate treated preparation with high NaK ATPase activity. There was no marked difference in reaction between the two types of membranes. To both types of membranes Mg 2+ gave a strong positive and ATP a slight negative addition to the membrane charge. In the presence of ATP Na + gave a higher charge contribution than did K + or a combination of Na + and K + . This implies that K + gives a higher affinity for ATP than Na + does and or that ATP mediates a higher affinity for Na + than for K + .