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Relative Contribution of Ribulose 1,5‐Diphosphate Carboxylase and Phosphoenolpyruvate Carboxylase to CO 2 Fixation Activity in Roots of Dark‐grown or Light‐grown Lens culinaris Seedlings
Author(s) -
NATO A.,
DELEENS E.
Publication year - 1975
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1975.tb03844.x
Subject(s) - phosphoenolpyruvate carboxylase , pyruvate carboxylase , carbon fixation , ribulose , phosphoenolpyruvate carboxykinase , rubisco , biochemistry , carboxylation , in vivo , chemistry , biology , enzyme , photosynthesis , botany , microbiology and biotechnology , catalysis
The pathway of carbon assimilation in greening roots was compared to the pathway in leaves of Lens culinaris seedlings by means of labelling distribution analysis among the products of 14 CO 2 fixation in vivo , and in vitro with ribulose 1,5‐diphosphate as the substrate. In green leaves, CO 2 fixation via ribulose 1,5‐diphosphate carboxylase predominated largely while, in green roots, this carboxylase activity and the phosphoenolpyruvate carboxylase contributed almost equally to the whole in vivo CO 2 fixation. A participation of the activities of both carboxylases according to the double carboxylation pathway in the synthesis of dicarboxylic acids (malate and aspartate) was demonstrated in vitro after 48 h of greening in roots but seemed to be absent in in vivo experiments.