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The Initiation and Maintenance of Vicia faba Tissue Cultures
Author(s) -
MITCHELL J. P.,
GILDOW F. E.
Publication year - 1975
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1975.tb03831.x
Subject(s) - kinetin , callus , explant culture , potassium nitrate , vicia faba , tissue culture , casein , radicle , hydrolysate , agar , botany , chemistry , nitrate , ammonium , food science , horticulture , biology , biochemistry , potassium , germination , hydrolysis , bacteria , in vitro , genetics , organic chemistry
Explants obtained by removing the radicle tip and the plumule from embryos of Vicia faba have been induced to form callus in culture. Of a range of agar‐solidified culture media tested, only that of Schenk and Hildebrandt (1972) was consistently successful. Improved growth, measured as increasing fresh weight was obtained by increasing the nitrogen content of the medium, either as potassium nitrate or as ammonium nitrate. A kinetin concentration of 0.01 mg/1 (5 × 10 −8 M ) and a 2,4‐dichlorophenoxyacetic acid (2,4‐D) concentration of 0.5 mg/1 (2.3 × 10 −6 M ) allowed optimum initial callus growth. A 2,4‐D concentration of 2.3 × 10 −8 M , while insufficient to induce callus formation was able to inhibit lateral root development which occurred from embryo explants cultured without added 2,4‐D. Subcultured tissue grew well on media supplemented with casein hydrolysate or a mixture of the eight most common amino acids in casein hydrolysate. Growth in subcultures was inhibited by two other amino acid mixtures used by other workers for different species.