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Embryoid and Plantlet Formation from Stock Cultures of Nigella Tissues
Author(s) -
BANERJEE S.,
GUPTA S.
Publication year - 1975
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1975.tb03829.x
Subject(s) - plantlet , callus , explant culture , murashige and skoog medium , botany , biology , tissue culture , hydrolysate , horticulture , chemistry , in vitro , biochemistry , hydrolysis
Embryogenesis could be induced within a short period in differentiated roots of Nigella sativa on solid Murashige and Skoog's medium containing casein hydrolysate (500 mg/1) and IAA (0.5 mg/1). Experimental root explants were taken from two different stocks: (i) previously differentiated from leaf callus and now being maintained through subcultures in liquid White's medium and (ii) freshly differentiated from leaf callus on solid Murashige and Skoog's medium + casein hydrolysate (100 mg/1) + IAA (0.5 mg/1). Fifty per cent of the embryoids produced 2n plantlets within 40–60 days.