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An Abscisic Acid‐like Substance in Dry and Soaked Phaseolus vulgaris Seeds Determined by the Lemna Growth Bioassay
Author(s) -
TILLBERG ELISABETH
Publication year - 1975
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1975.tb03819.x
Subject(s) - bioassay , abscisic acid , phaseolus , lemna , botany , chromatography , biology , chemistry , biochemistry , ecology , gene
An acid inhibitor, probably abscisic acid (ABA) in low concentrations was found to be present in bean seeds. The evidence is based on data from paper electrophoresis, chromatography, UV absorption and growth inhibition in the Lemna bioassay, sensitive down to a concentration of 10 ‐11 M ABA or 0.02–0.03 ng/flask. The inhibitor level as measured by this bioassay decreases considerably with increasing soaking time. Acid ether‐soluble inhibitors could even be detected in the soaking water from the soaked seeds.