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Chlorophyll Accumulation, CO 2 Fixation Enzymes, and Hill Activity, in Greening Roots of Lens culinaris
Author(s) -
NATO A.,
DELEENS E.
Publication year - 1975
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1975.tb03804.x
Subject(s) - greening , ribulose , seedling , rubisco , chlorophyll , biochemistry , pyruvate carboxylase , photosynthesis , chloroplast , chemistry , enzyme , amyloplast , carbon fixation , botany , biology , plastid , gene , ecology
The carboxylation of ribulose‐1,5‐diphosphate was demonstrated in vitro with extracts of ctiolated seedling roots. The presence of ribulose‐1,5‐diphosphate carboxylase was characterized in the subcellular fraction enriched in amyloplasts. Synthesis of chlorophyll, development of CO 2 fixation capacities and of Hill activity upon illumination have been studied with roots of Lens culinaris seedlings. The marked increases in CO 2 fixation with ribulose‐1,5‐diphosphate as the substrate and in Hill activity that occur after a lag phase seem to be related to cytological changes during the greening of roots.