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Morphogenesis in Tissue Cultures of Different Organs of Nigella sativa
Author(s) -
BANERJEE S.,
GUPTA S.
Publication year - 1975
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1975.tb03790.x
Subject(s) - callus , biology , ranunculaceae , auxin , botany , morphogenesis , shoot , nigella sativa , tissue culture , in vitro , traditional medicine , biochemistry , medicine , gene
Calli were isolated from root, stem and leaf segments of Nigella sativa (Fam. Ranunculaceae) on White's medium containing napthaleneacetic acid and coconut milk. From all the three types of calli, roots were differentiated. Shoot development occurred in stem and leaf calli only after the omission of first coconut milk and then of auxin from the basal medium and also in IAA (2.0 mg/ml) and coconut milk (15 % v/v) in the medium. Frequency of organ formation and the maintenance of this capacity depend upon the nature as well as the age of the callus tissue.