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Cell Wall Solubilization in Pedicel Abscission of Begonia Flower Buds
Author(s) -
GATE CH. H. HÄNISCH TEN,
NETTEN J.,
DORTLAND J. F.,
BRUINSMA J.
Publication year - 1975
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1975.tb03168.x
Subject(s) - abscission , pedicel , begonia , cell wall , cycloheximide , pectinase , pectin , ethylene , biology , explant culture , middle lamella , enzyme , botany , biochemistry , in vitro , protein biosynthesis , catalysis
Effects of metabolic inhibitors and growth regulators on the course of abscission and on the activities of cell wall solubilizing enzymes were studied in pedicel explants of Begonia flower buds. Actinomycin D, chloramphenicol and 2,4‐dinitrophenol slightly retarded abscission, whereas cycloheximide exerted a strong inhibition if applied until 10.5 h after explant excision. Indoleacetic acid retarded and ethylene promoted abscission and cell wall solubilization. However, the activities of cell wall solubilizing enzymes did not correspond with the course of abscission. No polygalacturonase and pectic acid and pectin transeliminases could be detected in the abscission zone during abscission, whereas a low pectin methylesterase activity did not change. Endo‐ and exocellulase activities did not increase until about 10 h after the onset of abscission, indicating that they are the result rather than the cause of abscission.