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Relative Activities of Some Cytokinin Fractions of Developing Cotton Fruit
Author(s) -
SANDSTEDT ROBERT
Publication year - 1974
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1974.tb05012.x
Subject(s) - cytokinin , fraction (chemistry) , chemistry , bioassay , callus , gossypium hirsutum , botany , chromatography , biology , auxin , biochemistry , gene , genetics
Abstract Ion‐exchange fractionization, paper chromatography, and soybean callus bioassay have partly resolved an apparent discrepancy in the sequence of cytokinin appearance in developing cotton ( Gossypium hirsutum L.) fruit compared to the sequence reported in corn ( Zea mays L.). Cotton fruit cytokinins were separated into three fractions: A combined n ‐butanol and ethylacetate extractable fraction comprised c . 10% of the cytokinin activity, a fraction not retained by a polymethacrylate weakly acidic cation‐exchange resin comprised c . 45%, and a fraction that was retained by the resin, c . 45%. The latter fraction differs from cytokinin fractions reported in corn. However, like cytokinin ribonucleotides, its cytokinin activity remains below R f 0.3 on paper chromatograms developed with water‐saturated sec ‐butanol, thus complicating studies of sequential cytokinin appearance in cotton. An anion‐exchange resin did not retain the cytokinin activity of this fraction.