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Compounds Association with Cytokinin Activity in Fruitlets and Tracheal Fluid of Gossypium hirsutum
Author(s) -
TAYLOR P. A.,
KOSUGE T.,
DeVAY J. E.
Publication year - 1974
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1974.tb05002.x
Subject(s) - callus , cytokinin , gossypium hirsutum , chemistry , adenosine , chromatography , botany , biochemistry , biology , auxin , gene
Compounds that account for cytokinin activity in fruitlets and tracheal fluids of cotton ( Gossypium hirsutum L.) were determined using preparations purified by thin‐layer chromatography and gas–liquid chromatography and bioassayed with cultures of soybean stem callus tissue. At least five substances were found that stimulated growth of callus tissue. Among these, adenine and adenosine accounted for most of the growth stimulation of callus tissue. A compound with cytokinin activity was isolated with TLC properties similar to other known cytokinins, but its identity as zeatin, dihydrozeatin or N 6 ‐(Δ 2 ‐isopentenyl)‐adenine was not confirmed using GLC and GLC combined with mass spectrometry. It was estimated that less than 1% of the cytokinin activity of this unknown substance could have been due to N 6 ‐substituted adenine derivatives.