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Partitioning of Cytokinins between Ethyl Acetate and Acid Water Phases
Author(s) -
HEMBERG TORSTEN
Publication year - 1974
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1974.tb03753.x
Subject(s) - kinetin , cytokinin , ethyl acetate , zeatin , chemistry , yield (engineering) , chromatography , riboside , biochemistry , in vitro , auxin , tissue culture , materials science , metallurgy , gene
Model‐experiments to determine the quantitative yield in purification of cytokinin extracts have been performed with kinetin, isopentenyladenine and zeatin and with their ribosides. When these substances in water solution of pH 3 are shaken three times with equal volumes of ethyl acetate, most of the kinetin and isopentenyladenine and their ribosides go over to the ethyl acetate phases. Zeatin and its riboside stay in the water phase. The unsuitability of using ethyl acetate to purify cytokinin extracts is pointed out.