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Effect of Age on Adenosine Triphosphatase Activity from Tobacco Chloroplasts
Author(s) -
SCHERINGS G.,
KUIPER P. J. C.
Publication year - 1974
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1974.tb03750.x
Subject(s) - chloroplast , adenosine triphosphatase , chemistry , adenosine , hydrolysis , botany , biochemistry , biology , atpase , horticulture , enzyme , gene
Adenosine triphosphatase activity of tobacco leaf chloroplasts in the dark was measured, using leaves of different age as determined by the position of the leaves along the stem. The activity of the chloroplast preparations strongly decreased with age, regardless of the addition of Mg 2+ or Ca 2+ . Opposite effects of Mg 2+ and Ca 2+ on the activity of the chloroplasts were noted in experiments where different ratios of Mg 2+ /Ca 2+ were applied. They were related to the age of the leaves, Ca 2+ strongly stimulated the activity of the preparations from old leaves but was practically without effect in young, just expanded leaves. Mg 2+ slightly stimulated the activity from old leaves while it invariably inhibited the hydrolytic activity of preparations from young leaves.