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Proteinase Inhibitors in Germinating Barley Embryos
Author(s) -
KIRSI MARKKU
Publication year - 1974
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1974.tb03732.x
Subject(s) - sephadex , germination , trypsin inhibitor , trypsin , biology , agar , embryo , size exclusion chromatography , seedling , agar plate , biochemistry , enzyme , botany , bacteria , genetics , microbiology and biotechnology
The activities of trypsin inhibitors and Aspergillus‐proteinase inhibitors have been assayed in barley embryos germinating in different conditions. The Aspergillus‐proteinase inhibitor activity increased about 20‐fold during 6 days at 20°C both in separated embryos germinating on nutrient agar and in the embryos of undissected grains germinating on agar with or without mineral salts. Significant inhibitor activity was also present in root tips at later stages of vegetative growth. Whether any changes occurred in the molecular weight distribution of the inhibitors present in the seedlings was studied by gel filtration on Sephadex G‐75; only minor changes were observed. The trypsin inhibitor activity per seedling remained roughly constant in all cultures. In gel filtration experiments all the activity appeared to be due to the inhibitor species present in resting embryos.