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Resistance and Sensitivity of Chloroplast Ribosomes to Streptomycin in Mutants of Chlamydomonas reinhardi
Author(s) -
BOSCHETTI ARMINIO,
NIGGLI VERENA,
OTZ URSULA,
WIEDMER THERESE
Publication year - 1974
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1974.tb03685.x
Subject(s) - chlamydomonas , streptomycin , ribosome , biology , thylakoid , chloroplast , biochemistry , protein biosynthesis , mutant , rna , gene , antibiotics
1 In a mendelian (sr 3 ) and an uniparental (sr 35 ) streptomycin resistant mutant of Chlamydomonas reinhardi the influence of streptomycin on protein synthesis on the chloroplast and cytoplasmic ribosomes was investigated in vitro . Hetero‐, mixo‐ and phototrophic agar cultures and heterotrophic liquid cultures were used. 2 Protein synthesis on the cytoplasmic ribosomes, measured by the activity of glyceraldehyde‐3‐phosphate: NADP dehydrogenase (EC 1.2.1.9), was not inhibited, but rather stimulated by streptomycin. 3 Protein synthesis on the chloroplast ribosomes of sr 3 , measured by the activity of ribulose‐1,5‐diphosphate carboxylase (EC 4.1.1.39), was greatly inhibited by streptomycin, especially in hetero‐ and mixotrophic cultures. In sr 35 the chloroplast ribosomes were resistant to streptomycin. 4 Heterotrophically grown cultures of sr 3 and of a streptomycin‐sensitive strain are yellow in the presence of streptomycin and form no or only reduced thylakoids on solid media. But 70‐S organelle‐ribosomes are present in a normal amount. 5 The relationship between chloroplast protein synthesis and thylakoid formation is discussed.