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Amino Acid Incorporation into Proteins by Plant Mitochondria in the Presence of Some Plant Growth Promoters, Metabolic Inhibitors and Synthetic Messengers
Author(s) -
DE DILIP K.,
ROY S. C.
Publication year - 1974
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1974.tb03676.x
Subject(s) - kinetin , ethidium bromide , biochemistry , biology , gibberellic acid , protein biosynthesis , dna , germination , tissue culture , in vitro , botany
The incorporation of 14 C‐labelled phenylalanine into proteins of mitochondria prepared from 48‐h germinating seeds of Vigna sinensis (L.) Savi can be stimulated by gibberellic acid (GA 3 ), kinetin and polyuridylic acid [poly(U)]. The incorporation is sensitive to various metabolic inhibitors which include rifampicin, actinomycin D, ethidium bromide, kanamycin, 5‐azauracil, 8‐azaadenine, and rotenone. They were used to study the effect of GA 3 and kinetin on the sequential steps in protein synthesis (DNA ‐> RNA ‐> protein), which are variously affected by the inhibitors. The inhibitory effect of actinomycin D on the incorporation can be reversed by GA 3 and kinetin, which implies that these two plant hormones act at the transcription level. With ethidium bromide the effect of hormones is not so convincing, though the trend is towards some release of inhibition. The plant hormones have apparently no effect on the incorporation sensitive to other inhibitors studied. GA 3 and kinetin can also stimulate the normal and polyadeny‐lic acid [poly(A)]‐directed incorporation of 14 C‐labelled lysine into proteins by a mitochondria! preparation, lndole‐3‐acetic acid and 2,4‐dichlorophenoxyacetic acid can only stimulate the normal synthesis, which is not further stepped up by poly (A). The polynucleotide even seems to depress the action of IAA. This anomaly has been discussed.