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Effects of Carbon Source, Gibberellins and Ethylene on Wall‐Bound Invertase Activity in Callus of Convolvulus arvensis
Author(s) -
KLIS F. M.,
GROOT C.,
VERWER R.
Publication year - 1974
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1974.tb03667.x
Subject(s) - invertase , sucrose , gibberellin , ethylene , chemistry , fructose , callus , incubation , biochemistry , enzyme assay , botany , enzyme , biology , catalysis
The growth rate of Convolvulus callus on a sucrose‐containing medium appeared to be largely independent of the activity of cell wall invertase. The increase of this enzyme activity which occurs upon subculturing was unaffected within the first 24 h by the presence of the substrate sucrose. Neither substitution by glucose or fructose, nor complete deletion of the carbon source had any effect. Gibberellins apparently were not involved in the initiation and/or control of the increase of wall‐bound invertase activity occurring upon subculturing. Exogenous ethylene was unable to mimick this effect of subculturing but when applied immediately after subculturing it had a synergistic effect on the increase of invertase. Inhibition occurred, however, when exposure to ethylene was delayed until after 24 h of incubation. These findings suggest a role of ethylene in the control of wall‐bound invertase activity.