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Protein and Ribonucleic Acid Synthesis by Plant Mitochondrial Systems
Author(s) -
GOSWAMI BISWENDU B.,
CHAKRABARTI SYAMALIMA,
DUBE D. K.,
ROY S. C.
Publication year - 1974
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1974.tb03137.x
Subject(s) - ethidium bromide , ribosome , biochemistry , mitochondrial ribosome , phenylalanine , transfer rna , mitochondrion , cytosine , chemistry , rna , uracil , protein biosynthesis , biology , amino acid , dna , gene
The incorporation of 14 C‐labelled phenylalanine into proteins of the mitochondrial systems obtained from 48‐h germinating seeds of Vigna sinensis (L.) Savi can be stimulated by polyuridylic acid [poly (U)] and depressed by rifampicin, which is, however, ineffective if poly (U) is allowed to interact with the incorporating system before the antibiotic has access to it. A system consisting of a mitochondrial S‐100 fraction and ribosomes from the same source with other cofactors can bring about polymerization of phenylalanine. The incorporation of 14 C‐labelled uracil into RNA by the plant mitochondria is greatly dependent on the exogenous addition of adenine, guanine, cytosine and also on 5‐phosphoribosyl‐l‐pyrophosphate (5‐PRPP). It is greatly suppressed by rifampicin and ethidium bromide.